Development and evaluation of doxorubicin self nanoemulsifying drug delivery system with Nigella Sativa oil against human hepatocellular carcinoma

摘要

Objective: The development of self nano emulsifying co-delivery system of doxorubicin and Nigella sativa oil for potentiating the anticancer effects against HepG2 cell lines. Materials and methods: SNEDDS were formulated by using Labrafil and N. sativa oil (3:2% w/w ), Kolliphor RH40 (15% w/w ), glycerol (5% w/w ) as oil phase, surfactant and co-surfactant while deionized water (75% v/v ) used as an aqueous phase. Optimized SNEDDS was evaluated for drug release and in vitro anticancer efficacy in liver cancer (HepG2) cell line. Results and discussion: The selected formulation (F6) has a mean particle size of 79.7?nm with PDI 0.098 and the minimum viscosity of 16.42?cps with % transmittance of 1.332 with maximum drug release of 96.968% in 32?h as compared to DOX alone. Stability data showed stable emulsion in both 25 0 C and -4 0 C.