Fluorometric Investigation on the Interaction of 5-Bromo and 5- Iodouracil with Bovine Serum Albumin

摘要

The Interaction of 5-Bromouracil (5BU) and 5-Iodouracil (5IU) with bovine serum albumin(BSA) were studied by spectroscopic method. Quenching of fluorescence of serum albumin bythese drugs was found to be a static quenching process. Based on the interaction studies carriedout the number of binding sites for drug on protein have been evaluated. The binding constant(Ka) were 2.013 x 104 and 4.062 x 104 L mol-1 for 5BU and 5IU.