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首页> 外文期刊>Annals of Agricultural Science >Optimization of biosurfactant production by Streptomyces isolated from Egyptian arid soil using Plackett–Burman design
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Optimization of biosurfactant production by Streptomyces isolated from Egyptian arid soil using Plackett–Burman design

机译:使用Plackett–Burman设计优化从埃及干旱土壤中分离的链霉菌的生物表面活性剂生产

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摘要

Surfactants are amphipathic molecules which reduce surface tension, widely used in pharmaceutical, cosmetic and food industries. The current study aimed to isolate Streptomyces species from Egyptian arid soil, efficient in biosurfactant production. Fifty isolates of Streptomyces were obtained from arid soil in Egypt. Primary screening, using lipase, drop collapse, parafilm M and haemolysis tests, showed 37 isolates (74%) having biosurfactant activity. Secondary screening resulted in 17 isolates based on their activities using emulsification index method. Isolate 5S was selected as the most efficient in biosurfactant production, having emulsification index equal to 31.74%. Plackett–Burman design was adopted to evaluate the factors affecting biosurfactant production. Among the tested nine variables of starch nitrate medium, treated molasses, peptone, Tween 80, incubation period and inoculum size were identified as significant factors affecting biosurfactant production. Cultivation of isolate 5S in the optimized medium elevated biosurfactant production to 42.68% (according to emulsification index) from 31.74%, obtained in un-optimized medium.
机译:表面活性剂是降低表面张力的两亲性分子,广泛用于制药,化妆品和食品工业。目前的研究旨在从埃及干旱土壤中分离链霉菌种,并有效地生产生物表面活性剂。从埃及的干旱土壤中获得了五十种链霉菌。初步筛选,使用脂肪酶,液滴崩解,parafilm M和溶血试验,发现37株(74%)具有生物表面活性剂活性。使用乳化指数法根据其活性进行二次筛选得到了17株分离株。分离物5S被选为生物表面活性剂生产中最有效的,其乳化指数等于31.74%。采用Plackett-Burman设计来评估影响生物表面活性剂生产的因素。在测试的9个变量中,硝酸淀粉培养基,处理过的糖蜜,蛋白ept,吐温80,潜伏期和接种量是影响生物表面活性剂生产的重要因素。在优化培养基中培养分离菌5S,可将生物表面活性剂的生产量从未优化培养基中的31.74%提高至42.68%(根据乳化指数)。

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