Sensitive Assay for Catecholamines in Pharmaceutical Samples and Blood Plasma Using Flow Injection Chemiluminescence Analysis

摘要

A rapid and sensitive chemiluminescence (CL) method using flow injection analysis was described for the determination of three catecholamines: dopamine, adrenaline and dobutamine, based on their greatly enhancing effects on the CL reaction of luminol-potassium periodate in basic solutions. Under the optimized conditions, the calibration graphs relating the increase of CL intensity to the concentration of the analytes were linear. The present method allows for the determination of dopamine, adrenaline, and dobutamine over the range of 1.0 × 10-10 - 1.0 × 10-7 g/ml. The relative standard deviations for measurements (n = 11) of dopamine, adrenaline and dobutamine were 2.9, 2.3 and 1.8% when the concentrations of three catecholamines were at 1.0 × 10-9 g/ml, respectively. The detection limits of the method were 2.0 × 10-11 g/ml dopamine, 1.0 × 10-11 g/ml adrenaline and 4.0 × 10-11 g/ml dobutamine. The method was successfully applied to the determination of three catecholamines in pharmaceutical samples and blood plasma.