Preparation of a molecularly imprinted fluorescent chemosensor using quinoline modified vinyl-β-cyclodextrin and acrylamide as monomers for the selective recognition of spermidine

摘要

A new molecularly imprinted fluorescent chemosensor with selectivity for spermidine has been developed using quinoline modified vinyl-?2-cyclodextrin as a reporter and major functional monomer. Acrylamide that provided synergetic monomer/template interactions was also used in the imprinting. The imprinted receptor of the chemosensor was created by the inclusion and hydrogen-bonding interactions between functional monomers and spermidine. The chemosensor has shown higher sensitivity and imprinting performance than that synthesized using only ?2-cyclodextrin. The imprinting factor of the imprinted membrane for spermidine is 3.87. This suggests that the imprinted chemosensor has good imprinted binding affinity. The selectivity (?±), evaluated by the ratio of sensitivity for spermidine to that for its analogs, was a‰§2.0, indicating the suitable recognition properties of the chemosensor. The fluorescence of the imprinted membrane exhibited a a€?Turn-ona€? response mode. A linear relationship between the negative logarithm of spermidine concentration and the fluorescence intensity of the chemosensor at Cspermidine of 5 ?— 10a?’7 to 1 ?— 10a?’4 mol La?’1 has been obtained. The results demonstrated that the use of vinyl-?2-cyclodextrin and radical polymerization for this chemosensor preparation has obvious advantages. The imprinted fluorescent chemosensor has application potential for spermidine determination in complex samples.