DETERMINATION OF THIOCYANATE IN HUMAN URINE SAMPLES BY SUPPRESSED ION CHROMATOGRAPHY

摘要

A sensitive and simple method has been developed for the determination of thiocyanate in human urine samples by suppressed ion chromatography with conductivity detector in which a carbonate solution mixed with trace amount of 1, 3, 5-benzenetricarboxylate (BTC) was used as an eluent. This eluent made thiocyanate elution earlier than a single eluent of carbonate, and also caused the peak of chromatogram for thiocyanate to be sharper When a 3mM carbonate -2μM BTC solution was used for a 50-μl aliquot of sample solution, the calibration graph obtained for thiocyanate plotted as peak height vs. concentration was linear up to 0.05mM, and the detection limit as S/N 3 was 0.2μM of thiocyanate. The proposed method was successfully applied to the determination of thiocyanate in various human urine samples.