An inkjet printing paper-based immunodevice for fluorescence determination of immunoglobulin G

摘要

A sequential inkjet printing strategy was utilized on a microfluidic paper-based fluorescence (FL) immunodevice for immunoglobulin G (IgG) detection. Assisted with four cartridges, capture antibodies, antigens, bovine serum albumin (BSA) and detection antibodies were sequentially printed on the paper surface with better reproducibility. For enzyme-linked immunosorbent assay (ELISA), the analysis time with inkjet printing on 10 paper-based devices can be reduced by about 4 min. IgG can be detected with a wide linear range and the detection limit was 0.4 ng mL?1. With the advantages of better repeatability, the size effect of gold nanoparticles (AuNPs) was also investigated using the inkjet printer's nozzle. As a result, particles with a diameter of less than 150 nm can be successfully printed on the paper surface without affecting the detection results. This work will provide a new strategy for time-saving and large-scale sample detection in the field of immunoassay.