Riboflavin production from mutants ofAshbya gossypii utilising orange rind as a substrate

摘要

The use of agroindustrial wastes such as orange rind as an alternative in the production of riboflavin was evaluated in this study withAshbya gossypii mutants.Ashbya gossypii mutants were obtained using 300 μg/mL of MNNG after an exposition period of 90 min with 2.3% of survival rate. A total of 11 mutant high yield strains of riboflavin were selected. Of these mutants, the most productive in YM medium were ASHLVII, ASHLX, ASHLXI and ASHLXV. Three additional different mutants were shown to be unsusceptible to inhibition by itaconate in the medium. When we used orange rind at 0.3% in YM medium without malt extract, the rate of riboflavin production was enhanced in the mutant strains producing 223 mg/L of this vitamin, an increase of 184% in the ASHLXI and ASHLXV mutants, as compared with the wild strain.