Power of different microsatellite panels for paternity analysis in sheep

摘要

The aim of this work was to develop and to test PCR-based suitable microsatellite marker panelsfor paternity testing in sheep. Blood samples were collected from 212 Kivircik sheep (24 ramsand their 188 offspring) raised in five farms were included in the controlled mating programmeand genotyped with 20 microsatellite markers. Different combinations with multiplex microsatellitegroups were established, providing seven microsatellite panels to determine the power of paternitytesting. A total of 318 alleles were detected across the 20 loci investigated. The average PIC value(0.80) obtained from all loci indicated that these microsatellite panels were very polymorphic andhighly informative. The highest and lowest average number of alleles were observed in Panel 3and Panel 1, respectively, among the formed panels. Probability of identity (PI) values varied from0.102 to 0.020. The power of discrimination (PD) values ranged from 0.678 and 0.979. Combinedprobability of identity (CPI) value ranged between 1.20x10-26 (Panel-7) and 7.24x10-6 (Panel-1). Thelowest and highest combined probability of exclusion values were recorded for Panel-1 (0.931912000)and Panel-7 (0.999998870), respectively, as it could have been expected. According to the studyresults panels 4 and 5, formed with fewer microsatellite markers for paternity tests, were cheaperand more practical than the other panels in Kivircik sheep.