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An Outbreak of Anthracnose Caused by Colletotrichum gloesporioides f.sp. manihotis in Cassava in North Western Tanzania

机译:炭疽菌引起的炭疽病暴发。坦桑尼亚西北部木薯中的manihotis

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Severe outbreak of anthracnose diseases caused by Colletotrichum gloesporioides f.sp. manihotis on cassava (Manihot esculenta Crantz) occured in north-western Tanzania in October, 2011. Thirteen cassava leaf samples with disease symptoms were collected from Maruku Agricultural Research Institute (MARI) and from farmers. The collected samples were tested for infection using Blotter method. The results indicated that, all 13 leaf samples (100%) were infected by C. gloesporioides f.sp. manihotis. Three isolates of the fungus per sample were subcultured on Potato Dextrose Agar (PDA) for seven days then each isolate was sprayed-inoculated onto 4-week-old sprouting leaves of cassava cuttings, var Mkombozi grown in pots in the screenhouse maintained at 27?C and relative humidity of 85% - 90%. All isolates were pathogenic on cassava and produced similar symptoms as those observed under field conditions. This is the first report of C. gloesporioides f.sp. manihotis a causal agent of anthracnose disease of cassava in Tanzania.
机译:由炭疽菌引起的炭疽病严重暴发。木薯上的木薯(Manihot esculenta Crantz)于2011年10月在坦桑尼亚西北部发生。从丸古农业研究所(MARI)和农民那里采集了13种具有疾病症状的木薯叶样品。使用Blotter方法测试收集的样品的感染。结果表明,所有13个叶片样品(100%)均被球孢梭菌感染。 manihotis。将每个样品的三种真菌分离物在马铃薯右旋糖琼脂(PDA)上传代培养7天,然后将每种分离物喷雾接种到4周龄的木薯插穗发芽叶片上,Mkombozi在筛房的盆中生长,保持在27℃。 C,相对湿度为85%-90%。所有分离株对木薯均具有致病性,并产生与田间条件下观察到的相似症状。这是C. gloesporioides f.sp.的首次报道。 manihotis是坦桑尼亚木薯炭疽病的病原体。

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