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Isolation and Identification of Enterobacteriaceae from Patients with Community Acquired Urinary Tract Infection

机译:社区获得性尿路感染患者肠杆菌科细菌的分离与鉴定

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The study was carried out in red sea state during the period from November 2013 to March 2014 to investigate the Enterobacteriaceae from patients suffering from community-acquired urinary tract infections and then do sensitivity test for each of isolates. In this study out of 100 specimens 52 Enterobacteriaceae species were isolated from different clinics and hospitals in Port Sudan city. The specimens werecultured on CLED media ( cystinelactose electrolyte deficiency). identification was done by gram's stain and convential biochemical reactions. Then the anti-microbial sensitivity tests were done as the follows: (Ampicillin-Sulbactam "AS", Co. trimoxazole "BA", Ceftizoxime "CI", Chloramphenicol "CH", Cephalaxin "PR", Tetracycline "TE", Ciprofloxacin "CP", Amikacin "AK", Sparfloxacin "SC", Gatifloxacin "GF", Norflaxcin "NX) by Kirby-Bauer disc diffusion method. The study revealed that the most part of strains are sensitive to Chloramphenicol andAmikacin and resistant to Ampicillin-Sulpactam (AS). The identified Enterobacteriaceae were as follows; Escherichia coli 34 (65%), Klebsiella pneumonia 10 (19%), Klebsiella oxytoca 3(6%), Salmonella Para A 3 (6%), proteus mirabilis 1 (2%), Citrobacter 1 (2%).
机译:该研究于2013年11月至2014年3月在红海州进行,以调查患有社区获得性尿路感染的患者的肠杆菌科,然后对每种分离株进行敏感性测试。在这项研究中,从苏丹港市不同诊所和医院中分离出100种标本中的52种肠杆菌科。将标本在CLED培养基(半胱氨酸半乳糖电解质缺乏)上培养。通过革兰氏染色和常规生化反应进行鉴定。然后进行如下抗微生物敏感性测试:(氨苄青霉素-舒巴坦“ AS”,曲美唑公司“ BA”,头孢唑肟“ CI”,氯霉素“ CH”,头孢拉辛“ PR”,四环素“ TE”,环丙沙星“ CP“,阿米卡星” AK“,司巴沙星” SC“,加替沙星” GF“,诺氟沙星” NX)通过圆盘扩散法研究。研究表明,大多数菌株对氯霉素和阿米卡星敏感,并对氨苄西林-舒帕坦具有抗药性(AS)。鉴定的肠杆菌科如下:大肠杆菌34(65%),肺炎克雷伯菌10(19%),产氧克雷伯菌3(6%),沙门氏菌A 3(6%),变形杆菌1(2%) ),柠檬酸杆菌1(2%)。

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