The Mechanisms of PLANT Rhizome Curcuma Longa Action on Carbohydrate Metabolism in Alloxan - Induced Diabetes Mellitus Rats

摘要

In experiments on rats with the alloxan-induced diabetes model the mechanisms of the turmeric (Curcuma longa) rhizomes influence on the various links in carbohydrate metabolism were studied. For this purpose, the glucose concentration in whole blood, titer hormones of insulin, C-peptide and cortisol in plasma, content of glycogen in the liver, structural and functional organization of the islet apparatus of the rats pancreas were studied. Under the influence of the turmeric rhizomes intake in rats with diabetes, as compared with the same animals on a standard diet, there were less expressed increasing of the glucose concentration in blood, increasing of the insulin and C-peptide concentration in plasma, lowering the concentration of cortisol and reliable increasing of the glycogen level in the liver. In the study of the microstructure of samples of pancreatic tissue of experimental animals on the background of the turmeric intake, the most preservation of the islet apparatus in comparison with a group of animals in conditions of a standard diet was marked. The results indicate the positive impact of the Curcuma longa rhizomes on the homeostatic mechanisms of the carbohydrate metabolism regulation in the alloxan-induced diabetes.Aim. The effects of the plant rhizome Curcuma longa as a food additive were studied on different processes of carbohydrate metabolism: glucose concentration in blood, concentration of hormones - insulin and C-peptide in plasma, content of glycogen in the liver, structural and functional organization of the islet apparatus of the pancreas in rats with alloxan-induced diabetes mellitus. Materials and methods. The concentration of glucose in blood and perfused solution was determined with the picricacid method by intensity of colour reaction on a spectrophotometer. The concentration of hormones (insulin, C-peptide) was determined by the immunoenzyme method with standard sets on a tablet spectrophotometer. The morphological structure of the pancreas was studied by a method of light microscopy. The content of glycogen in the liver was measured by means of a SHICK-reaction on the Mac-Manus method with measurement of colour intensity on a spectrophotometer. Results. Under the influence of the turmeric rhizomes intake in rats with diabetes, as compared with diabetic animals on a standard diet, a lower increase of the glucose concentration in blood, the tendency of decrease of glucose absorption in the gut, higher concentration of the insulin and C-peptide in plasma and the significant increase of glycogen content in the liver was found. The microstructure of pancreatic tissue samples of experimental animals using turmeric intake, was characterized by the better preservation of the islet apparatus in comparison with a group of animals on a standard diet. Conclusion. The results indicate the positive effect of the Curcuma longa rhizomes on the homeostatic mechanisms of the carbohydrate metabolism regulation in the alloxan-induced diabetes mellitus rats.