Quantitative Study of the Evaluation of the Activity of Immune Cells in the Spleen of Diseased Mice with Cancer by Soft Laser

摘要

Background: Little informations have been published about the active role of soft laser in stimulation the immune cells, so, our work was conducted to examine experimentally the capacity of the immune cells such as macrophages of the spleen in reducing the tumor size of diseased mice with mammary gland carcinoma by soft laser. Materials and Methods: Fourty healthy female Swiss albino mice aged two months (weighing 45-48g) were used in the present experimental work. They were divided into five groups of six mice each. All the animals inoculated with mammary gland carcinoma. After successful inoculation, the animals were anaesthetized and irradiated with laser for 25 minutes with different time intervals and for ten days. The tumor size was measured before and after irradiation by vernier, daily, starting from tumor appearance until the last day of the experiment (10days). Sections of spleen were prepared and examined for histopathological study by light microscope. Results: The present experimental work showed reduction in tumor size of diseased mice with carcinoma irradiated with laser with the increasing of time interval for each experimental group of mice when compared to the tumor size of diseased mice with carcinoma not irradiated with laser. The histological examinations of the sections of the spleen of the diseased mice with carcinoma irradiated with laser showed abundant histological alterations in the structure of the macrophages such as increased size and multiplication of the nucleus when compared to the macrophages of the spleen of the diseased mice with carcinoma not irradiated with laser. Conclusion: The present experimental work proved that soft laser played an important role in activation the immune cells such as macrophages of the spleen and then decreased the tumor size of diseased mice with carcinoma and caused marked histological alterations in the structure of the macrophage due to laser action.