EXTRACTION AND IDENTIFICATION OF A SERINE PROTEASE INHIBITOR FROM FLAX SEED (Linum usitatissimum L.)

摘要

The trypsin inhibitor from common flax seeds (Linum usitatissimum trypsin inhibitor, abbreviate LUTI) is a member ofthe potato inhibitor I family. This was isolated and purified by ethanol fractionation, ion exchange chromatography andaffinity chromatography. To determine the molecular mass of protease inhibitor we utilized gel electrophoresis indenatured conditions (SDS-PAGE). LUTI is the first serine protease-type inhibitor isolated from a plant of Linaceaefamily, and has a molecular mass 7000D.