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Seed Quality Evaluation by Tetrazolium Staining of &i&Parkia multijuga&/i& Benth

机译:通过Parkia multijuga / i的四氮唑染色评价种子质量。贝思

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The purpose of this study was to develop a staining pattern through tetrazolium test to evaluate seed viability of Parkia multijuga Benth (fava-arara-tucupi). The seeds were scarified, soaked into water and distilled for 48 hours for tegument removal and then immersed in a 0.05%; 0.1%; and 0.3% tetrazolium solution for 6; 18 and 24 hours at 35 ° C, in the dark. The evaluation was performed on the basis of stain intensity and aspect of the tissues. Based on color patterns, the seed images were classified in eight viability classes. Those results were compared with the germination test in a laboratory. The treatment in concentration of 0.1% for 6 hours was considered adequate for the specie, considering the adequate staining and time for analysis.
机译:这项研究的目的是通过四氮唑试验建立一种染色模式,以评估Parkia multijuga Benth(fava-arara-tucupi)的种子活力。将种子弄碎,浸入水中并蒸馏48小时以去除皮层,然后浸入0.05%的溶液中。 0.1%; 0.3%四唑鎓溶液中的6;在黑暗中于35°C放置18和24小时。根据染色强度和组织的外观进行评估。根据颜色模式,将种子图像分为八个生存能力类别。将这些结果与实验室中的发芽测试进行了比较。考虑到足够的染色和分析时间,认为以0.1%的浓度处理6小时对于该物种是足够的。

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