Artemisia cina is a medicinal plant species which produces bioactive compound potential to anti-tumor, antifungal and antibacterial medicines. This study was aimed to obtain A. cina polyploid plants through a treatment of growth regulators in shoot culture. The shoot were treated in 1; 15; 2; and 3 mg L -1 of 2,4-dichloro phenoxy acetic acid (2,4-D) combined with 1; 1.5; 2; 3 mg L -1 of Benzyladenine (BA) for 28 days. Chromosome analysis showed that the highest polyploidy percentage, 23%, was reached in the treatment of 2 mg L -1 of 2,4-D combined with 1 of BA mg L -1 . The polyploidy level varied, comprising 2n=3x, 2n=4x, 2n=5x, 2n=6x, with the highest polyploidy level percentage, 28.57%, and it was attained in the tetraploid (2n=4x). Polyploid plants had larger leaves area, larger stomatal size, and higher chlorophyll content than diploid plants. However stomatal density of polyploidy plants was lower than that of in diploid plants.
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机译：青蒿是一种药用植物，可产生具有抗肿瘤，抗真菌和抗菌作用的生物活性化合物。这项研究的目的是通过处理芽培养中的生长调节剂来获得中华曲霉多倍体植物。芽处理1。 15; 2; 3mg L -1的2,4-二氯苯氧基乙酸（2,4-D）与1的混合物; 1.5; 2; 3 mg L -1的苯甲醛（BA）持续28天。染色体分析显示，在2 mg L -1的2,4-D与1 BA BA的L -1组合中，最高的多倍体百分比达到23％。多倍体水平变化，包括2n = 3x，2n = 4x，2n = 5x，2n = 6x，多倍体水平百分比最高，为28.57％，并且在四倍体中达到（2n = 4x）。多倍体植物比二倍体植物具有更大的叶面积，更大的气孔大小和更高的叶绿素含量。然而，多倍体植物的气孔密度低于二倍体植物的气孔密度。
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