Optimised production of L-glutaminase: A tumour inhibitor from Aspergillus flavus cultured on agroindustrial residues

摘要

L-Glutaminase, an amidohydrolase enzyme has been a choice of interest in the treatment of lymphoblastic leukemia. This?study?investigates?the production and optimization of?extracellular glutaminase?enzyme using several agro-industrial residues by?Aspergillus flavus?KUGF009?using SSF (solid state fermentation).Effect of process variables namely substrates, incubation period, temperature, moisture content, initial pH, supplementary carbon and nitrogen sources and metal ions on the production of L-glutaminase?was?studied and accordingly, optimum conditions?were?determined.?A. flavus?KUGF009??was cultured in tea dust to produce L-glutaminase. The organism produced high levels of glutaminase under optimized culture conditions on?the?5th day of incubation at an optimum pH 4.0, temperature 30°C and moisture content 50% by SSF. Enhanced production occurred on?the?addition of dextrose, yeast extract and MgSO4?as nutritional factors.