Determination of mycoflora of pea (Pisum sativum) seeds and the effects of Rhizobium leguminosorum on fungal pathogens of peas

摘要

Mycoflora of pea seeds and the effectiveness of?Rhizobium leguminosarum?on important seed-borne pathogens were determined. Mycoflora were changed according to seed groups with or without surface sterilization. The most common isolated fungi were?Fusarium?spp.,?Alternaria?spp.,?Macrophomina phaseolina, Phytophthora megasperma,?Rhizoctonia solani?and?Sclerotium rolfsii.?R. leguminosarum?showed antagonistic effect on?Fusarium oxysporum?and?P. megasperma?by 66.3 and 62.1%, respectively, in dual culture test?in vitro.?R. leguminosarum?application reduced disease severity of?P. megasperma,?R. solani,F. oxysporum?and?S. rolfsii?by 52.9, 50, 67.9 and 52%, respectively. Results indicated that?R. leguminosarum?showed effects on plant pathogenic fungi and could be used as biocontrol agent.