Isolation and molecular characterization of DgZFP2: A gene encoding a Cys2/His2-type zinc finger protein in chrysanthemum

摘要

The plant Cys2/His2-type zinc finger protein genes play critical roles in response to abiotic stresses. However, the roles of the chrysanthemum Cys2/His2-type zinc finger protein genes in plant stress responses are still not well known. In the present study, a full-length cDNA designated?DgZFP2, containing two conserved Cys2/His2-type zinc finger motifs with a plant-specific QALGGH motif in each zinc finger domain, a L-box (EXEXXAXCLXXL), and an EAR-box (DLNL) at C-terminus, was isolated from chrysanthemum by rapid amplification of cDNA ends (RACE).?It encodes a protein of 178 amino acids residues with a calculated molecular mass of 20.05 kDa and theoretical isoelectric point of 8.24. The transcript of?DgZFP2?was enriched in flowers and leaves than in roots and stems of the adult chrysanthemum plants. The gene expression was strongly induced by NaCl, drought, cold, and abscisic acid (ABA) treatment in the seedlings. We argued thatDgZFP2?is a new member of the Cys2/His2-type zinc finger protein genes, and it may be involved in the abiotic-stress response in plants.