Zinc Finger Protein (PFE) isolated artificial isolation, fusion protein, Nucleic Acid, expression vector, host Cell, a method for preparing a zinc Finger Protein, method for preparing Nucleic Acid method to design one or more domains of zinc finger, method for dis

摘要

A zinc Finger Protein (PFE) artificial Isolation to be linked to a Target sequence of nucleic acids, comprising at least three zinc finger domains covalentmente Together through 0 to 10 amino acid residues where the amino acids are in the position - 1 2, 3, and 6 of alpha Helix, zinc finger, selected as follows: at position - 1It is the amino acid arginine, glutamine or glutamic acid, threonine, methionine, the amino acid at position 2 is Serine, asparagine, threonine or Aspartic acid in position 3, is the amino acid asparagine, histidine, Aspartic acid, or would be, and the amino acid at position 6 is Argin INA, threonine, tyrosine, glutamine,Leucine and glutamic acid; or comprises at least three zinc finger Domains, each of the zinc finger Domains, independently, is represented by the formula: - - - - 4 x2 X3 Cys - Cys X5 z 1 - X - - - - - - Z2 Z3 Z6 X2 - His - - His - - 5 X3 x4, these domains are independently co Bravely together with 0 to 10 amino acid residues; X is independentlyAn amino acid, and Xn is the number of times x occurs in the polypeptide Chain; Z - 1 is arginine, glutamine or glutamic acid, threonine, methionine, Serine, asparagine, threonine is Z2, Z3 is Aspartic acid or asparagine, histidine, Aspartic acid, Serine or Z6 e; and S arginine, threonine, tyrosine, glutamine,Leucine and glutamic acid; provided that in each case the ZEP has an Amino Acid Sequence comprising any of SEQ ID nos.3 - 12; isolated fusion proteins with domains of PFE under this, Nucleic acids and Expression Vectors encoding these zfps and Fusion Proteins, host cells and Methods for preparing the zfps and Fusion Proteins, Methods for preparing Nucleic Acid Methods to design one or more domains of zinc finger,Methods to design a multi Protein zinc finger Domains, Methods to link a Nucleic Acid target with an artificial zinc Finger Protein, Methods for Linking a Nucleic Acid target with a zinc Finger Protein of Multiple domains, Methods for Modulate the expression of a Gene, Methods for altering the structure GenomicsMethods for inhibiting viral Replication, Transposasas artificial methods to a specific target for the introduction of an exogenous Gene In The genome of an organism, Methods to split specific for a target of an exogenous Gene from the Genome of an organism, Methods P To detect an altered sequence of zinc finger Recognition,Methods to Diagnose a disease associated with abnormal genomic structure, sets of oligonucleotides oligonucleotides separated sets of 256 256 goals designed to target the 4 base Pairs and allow the production of All Three Fingers (zfps is possible Cir, 10 (6) of a total of 768 oligonucleotides, equipment,Mono - and oligo or bifilamentos.