Effect of Alcoholic Extract of Salvia officinalis Leaves on some Physiological Parameters Aspects in Acrylamide-Treated Rats

摘要

This study was carried out to explore the effects of alcoholic extract of Salvia officinalis (SO) leaves on some physiological parameter including: protein profile, antioxidant status,some immunological and proinflammatory biomarkers in acrylamide (ACR) comparing to cyclosporine (Cys) treated rats. Thirty adult male rats were selected randomly and equally allocated into six gropes and treated daily for 45 days with oral manner as follow: Group G1: (control group) received tap water), G2: rats in this group were received (150 mg/kg /B.W) of alcoholic extract of SO leaves,G3: rats of this group were received (1 mg/kg /B.W) of acryl amide in drinking water, G4: rats in this group were received (1mg/kg /B.W) of cyclosporine, G5: rats in this group were received (150 mg/kg /B.W) of Salvia officinalis leaves extract and acryl amide(1 mg/kg /B.W), G6: rats in this group were received the same mentioned dose of SO and cyclosporine as in G2 and G4 groups. At the end of experiment, animals were sacrificed and blood was drawn by cardiac puncture technique for measuring the following parameters: serum reduced glutathione (GSH), peroxynitrite radical, total protein, albumin, globulin concentrations as well as phagocyte index (%) and mitotic index (%). Serum immunoglobulin G (IgG), interleukin-6 (IL-6) and C – reactive protein (CRP) concentrations were also recorded. The results of the current study revealed the beneficial effect of alcoholic extract of Salvia officinalis against deleterious effect of ACR and cyclosporine illustrated by its antioxidant, immunostimulatory and anti-inflammatory effect. Significant increase in serum TSP, albumin and globulin concentration, as well elevation in serum GSH and depression in peroxynitrite radical concentration was observed after oral intubation of SO alone or in combination with ACR or Cys comparing to the ACR or cyclosporine treated rats which showed controversial result. Concerning immune-stimulatory and anti-inflammatory effect of Salvia officinalis, significant elevation in phagocytic index % (PI), mitotic index % (MI) and in serum (IgG), as well as significant depression in serum IL-6 and CRP concentrations was observed following extract intubation comparing to the immune-supperion and pro inflammatory effect of ACR and cyclosporine. On conclusion, alcoholic extract of Salvia officinalis (SO) leaves alleviate the detrimental effect of ACR and cyclosporine correlated with their pro oxidative, immune suppressive and pro-inflammatory effect. Non-significant differences between ACR and Cys were observed in all measure issue.