Detection of Group A Rotavirus in Faeces of Diarrhoeic Bovine Porcine and Human Population from Eastern India by Reverse Transcriptase-Polymerase Chain Reaction

摘要

The present study describes the distribution of group A rotavirus in animals and human population of eastern state of India– West Bengal. During the study, a total of 211 samples were collected from diarrhoeic bovine, porcine and human population (below 6 months of age). Of which , 26 (12.32%) samples were found positive for group A rotavirus by VP6 gene based reverse transcriptase– polymerase chain reaction (RT–PCR) assay. A total of 89 bovine, 82 porcine faecal and 40 human stool samples were screened by RT–PCR assay for the detection of group A rotavirus, of which 10 bovine (11.23%), 9 porcine (10.97%) and 7 human (17.5%) samples were detected positive. The study revealed baseline information to understand rotavirus epizootology and future prophylactic strategies against rotavirus in eastern India