A New Commercial Protocol for Micropropagation of Myrtus tree

摘要

This research was conducted in 2013, in order to propagation of Myrtus communis tree via tissue culture technique. In this study, the rate of shoot proliferation and rooting were evaluated. The results indicated that between, MS (Murashige & Skooge 1962) and WPM ( woody plant Medium), media ,WPM showed better result rather than MS. In Shoot proliferation stage were used benzyl adenine(BA) at levels of 0, 0.5, 1, 2, 3, 4, 5 and 6 mg /l and indole butyric acid(IBA)at levels of 0 and 0.1 mg /l, then three traits containing: leaf number, shoot length, and shoot proliferation were examined. The best result was obtained by using of modified WPM medium with BA in 4and IBA in 0.1mg/l. The mean of shoot in this treatment was 40 shoots in each tube. For rooting, MS medium was used which adjusts IBA in 0, 1, 2, 3, 4, 5 mg/l that the best result was obtain by using of 3 mg/l IBA and dark period of 8 days. For adaptation stage of this plant, two contexts were used; jiffy pots as well as combination of 60% washed sand with 40% of peat moss which was successful in both contexts.