Optimized Preparation of Catechin Nanoliposomes by Orthogonal Design and Stability Study

摘要

Catechin nanoliposomes are prepared by reverse-phase evaporation method. Taking the entrapment efficiency of catechin nanoliposomes as the main evaluation index, the prescription of the catechin nanoliposomes was optimized by orthogonal design. Found by experiment, the orthogonal design test showed that phosphatidylcholine to cholesterol ratio had the highest influence in preparation and rotary evaporation temperature the lowest, with catechin concentration and ion strength in between. The best conditions of preparation of catechin nanoliposomes are that phosphatidylcholine to cholesterol ratio of 4, catechin concentration of 4.00 mg/mL, ion strength of 0.02 mol/Landrotary evaporation temperature of 40°C, encapsulation efficiency can reach about 53.69±1.65%, the particle size is 235±4 nm. Finally, using pH, leakage ratio and size to explore the stability, catechin nanoliposomes showed an acceptable stability.