Background. The aim of this study was to determine the best extraction conditions for total phenolic content (TPC) and antioxidant capacity (AC) of Polygonum multiflorum Thunb. root using microwave-assisted extraction (MAE).Material and methods. The raw material used was Polygonum multiflorum Thunb. root powder. Five factors such as solvent type, solvent concentrations, solvent/material ratio, extraction time and microwave power were studied; TPC and AC values were determined by the Folin-Ciocalteu method and DPPH free radical scavenging activity measurement, respectively. In addition, studies involved assaying the HPLC test of extracts and SEM of samples.Results. Optimal results pointed to acetone as the solvent, acetone concentration of 60%, solvent/material ratio of 40/1 (v/w), extraction time of 5 mins and microwave power of 127 W. TPC and AC obtained were approximates 44.3 ±0.13 mg GAE/g DW and 341.26 ±1.54 μmol TE/g DW, respectively. The effect of microwaving on the cell destruction of Polygonum multiflorum Thunb. root was observed by scanning electron microscopy (SEM). Some phenolic compounds were determined by the HPLC method, for instance, gallic acid, catechin and resveratrol.Conclusion. These factors significantly affected TPC and AC. We can use acetone as a solvent with microwave-assisted extraction to achieve the best result.
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机译：背景。这项研究的目的是确定何首乌总酚含量（TPC）和抗氧化能力（AC）的最佳提取条件。使用微波辅助提取（MAE）根。材料和方法。使用的原料是何首乌。根粉。研究了溶剂类型，溶剂浓度，溶剂/物料比，萃取时间和微波功率等五个因素。 TPC和AC值分别通过Folin-Ciocalteu方法和DPPH自由基清除活性测量来确定。此外，研究还涉及分析提取物的HPLC测试和样品的SEM。结果。最佳结果表明，以丙酮为溶剂，丙酮浓度为60％，溶剂/物料比为40/1（v / w），萃取时间为5分钟，微波功率为127W。获得的TPC和AC约为44.3±0.13 mg GAE / g DW和341.26±1.54μmolTE / g DW。微波对何首乌细胞破坏的影响。通过扫描电子显微镜（SEM）观察根。通过HPLC方法测定了一些酚类化合物，例如没食子酸，儿茶素和白藜芦醇。这些因素显着影响了TPC和AC。我们可以使用丙酮作为溶剂，微波辅助萃取，以达到最佳效果。
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