Enterocin 4231 produced by Enterococcus faecium CCM 4231 and its use in rabbits

摘要

The aim of this experiment was to study the effect of Enterocin (Ent) 4231 produced by Enterococcus faecium. The group of 48 rabbits (Hy-Plus breed) was divided into experimental (EG) and control (CG) group. In EG Ent 4231 was applied for 21 days in a dose of 50 μL/animal/day into drinking water. The experiment lasted for 42 days. Samples were collected on day 0-1, day 7, day 21 (3 weeks of administration) and on day 42 (3 weeks after cessation of application) to measure the occurence of Eimeria sp. oocysts. Samples of caecal contents were collected on days 21 and 42 to determine bacterial counts. The phagocytic activity was examined on days 0-1, 21 and 42. On days 21 and 42 the reduction of Clostridium-like sp. was detected in EG (difference 1.53 log10 CFU/g; 1.57 log10 CFU/g) in comparison with CG. In EG on day 7 the decrease of E. coli (difference 1.30 log10 CFU/g) was also noted in comparison with CG. In EG prolonged immunostimulative effect (p<0.001) was observed on day 42 in comparison with CG. The oocysts of Eimeria sp. were reduced in EG comparing with CG at day 42. Enrichment of the diet for rabbits with Ent 4231 did not influence biochemical, zootechnical parameters and the values of caecal content volatile acids during the whole experiment.