A sensitive, simple and rapid spectrophotometric procedure for the assay of trace quantities of sulfadiazine (SDZ) drug as bulk and in diluted solution is characterized. The procedure depends on the diazotization reaction which is produced by coupling SDZ with (4-amino-2-hydroxy acetophenon) (AHA) to produce an intense colored complex spectrophotometrically determined at 410 nm. Beer’s law was applied in the range of concentration 0.5 - 15 ppm; the molar absorptivity and Sandell’s sensitivity were 2.8484 × 104 L mol-1 cm-1 and 0.008 μg cm-2 respectively. The method limit of detection (LOD) was 0.443 μg mL-1 and LOQ (the method limit of quantitation) was 0.249 μg mL-1. The procedure is not based on solvent extraction and the additives and excipients do not significantly influence the developed procedure.
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机译：表征了一种灵敏，简单，快速的分光光度法，用于分析痕量的磺胺嘧啶（SDZ）药物（散装和稀释溶液）。该程序取决于重氮化反应，重氮化反应是通过将SDZ与（4-氨基-2-羟基苯乙酮）（AHA）偶联产生的，通过分光光度法在410 nm处测定，产生强烈的有色络合物。适用比尔定律，浓度范围为0.5-15 ppm;摩尔吸收率和Sandell灵敏度分别为2.8484×104 L mol-1 cm-1和0.008μgcm-2。方法的检测极限（LOD）为0.443μgmL-1，LOQ（定量的方法极限）为0.249μgmL-1。该程序不是基于溶剂提取的，添加剂和赋形剂不会显着影响开发的程序。
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