In vitro propagation of Heliconia bihai (L.) cv. Lobster Salmón from flowering buds

摘要

A protocol for in vitro propagation of Heliconia bihai cv. Lobster Salmón was developed using flowering buds as initial explants. 250 flowering buds were established in different assays with NaClO at 1% during 20 minutes. The establishment in Murashige & Skoog's medium with BAP (2mg/L), AIA (1 mg/L) and L-cystein (0.1 g/L) showed the best results. Shoot multiplication was obtained with BAP (6 mg/L) with a subsequent subculture in a medium with BAP (2 mg/L) and AIA (0.5 mg/L), reaching multiplication rates of 3.0 new shoots. Rooting was achieved with AIA 1.3 mg/L. The survival rate during greenhouse acclimatization was 92%. Analysis of variance (ANOVA) and Tur