首页> 外国专利> In vitro propagation of Heliconia bihai (L.) CV. Lobster and salmon Heliconia orthotricha CV. Rainbow

In vitro propagation of Heliconia bihai (L.) CV. Lobster and salmon Heliconia orthotricha CV. Rainbow

机译:Heliconia bihai(L.)CV的体外繁殖。龙虾和三文鱼Heliconia orthotricha CV。彩虹

摘要

The present Invention relates to in vitro propagation from floral Meristem of Heliconia bihai CV. Lobster and salmon Heliconia orthotricha CV. Rainbow. Floral buds were established using 1% for 20 minutes was born and grown in Murashige and Skoog Medium containing 2mg / l BAP, IAA 1mg / L and 0.1 g / l of L - cysteine.L amultiplicaciu00f3n outbreaks was achieved using 6 mg / l BAP and subsequently a Medium with 2 mg / l BAP and iba 0.5 mg / l, reaching a rate of multiplication of 3.0. The rooting of in vitro plantlets was obtained using 1.3 and 20 mg / L iba. In the Acclimatization Nursery achieved a 92% Survival.The spread of heliconias from floral buds require more time in the phase of Establishment and multiplication but allow larger percentages of survival and less pollution, providing promising results to be applied to a number of heliconias. Commercial interest.
机译:本发明涉及从Heliconia bihai CV的花分生组织进行的体外繁殖。龙虾和三文鱼Heliconia orthotricha CV。彩虹。用1%的花蕾建立20分钟的花蕾,并在Murashige和Skoog培养基中生长并生长,其中含有2mg / l BAP,IAA 1mg / L和0.1g / l L-半胱氨酸。 6 mg / l BAP,然后添加2 mg / l BAP和iba 0.5 mg / l的培养基,达到3.0的繁殖率。使用1.3和20mg / L iba获得离体幼苗的生根。在适应环境中,苗圃的存活率达到92%。从花蕾中传播的Heliconias在建立和繁殖阶段需要更多的时间,但可以实现更大的存活率和更少的污染,可应用于许多Heliconias。商业利益。

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