In vitro Propagation of Citrus limonia Osbeck Through Nucellar Embryo Culture

摘要

Citrus limonia Osbeck is a promising rootsctock for commercial citrus species with sturdy and disease and drought resistant characters. A n efficient and highly reproducible plant regeneration protocol has been developed from nucellar embryo of Citrus limonia. Murashige and Skoogs medium was used for plant regeneration from nucellar embryos. It was noted that 6-benzylaminopurine at a concentration of 2.22 mM induced highest number of multiple shoots as 18.26 shoots per explant. On transfer of individual shoots to root inducing MS medium supplied with auxins, Indole-3-butyric acid (2.46 μM) and 6-benzylaminopurine (1.11 μM) proved to be the best combination. This media resulted in 78.80 % rooting and produced plantlets with an average of 5.53 roots/ shoot. Plantlets thus obtained were successfully transferred to green house for further observations. The micropropagated plantlets thus obtained can be used as rootstocks by traditional method or by micrografting.