Mamu -DQA1 Allele and Genotype Frequencies in a Randomly Sampled Breeding Colony of Rhesus Macaques (Macaca mulatta)

摘要

Westudiedtheallelicandgenotypicdistributionofthemajorhistocompatibilityclass-IIlocusDQA1observedinarandomsampleofIndianrhesusmacaques(Macacamulatta)fromamajorbreedingfacilityintheUnitedStates.TheDNAwasisolatedfromwholebloodsamplescollectedbetween1991and1994from65Indianrhesusmonkeys.Polymerasechainreaction-restrictionfragmentlengthpolymorphismanalysis(PCR-RFLP),whichinvolvesuseofspecificamplificationofDQA1exon2andsubsequentrestrictiondigestionofthe242-basepairfragment,wasusedtogenotypetheanimalsforthe20knownmacaque(Mamu)-DQA1alleles.Frequenciesforfouralleles(DQA1*240x,*2502,*2503and*0102)differedsignificantlyfromthosereportedinasmallersampleofrhesusmacaquesfromtheGermanPrimateCenter.ThemodestgeneticsurveyofMamu-DQA1genotypespresentedherewillbeparticularlyusefulindesigningepidemiologicstudiesthatinvestigateassociationsbetweenimmunogeneticbackgroundanddiseasesusceptibilityinmacaquemodelsofhumandisease.