Minimally Invasive Surgery via Laparoscopy for Intra-Abdominal Biopsy in Obese Rhesus Macaques (Macaca mulatta)

摘要

Purpose.Todeterminethesafetyandeffectivenessoflaparoscopyforrepeatedintra-abdominalbiopsyofliverandomentaladiposetissue(AT)inobeserhesusmonkeys(Macacamulatta).brxmlns="http://pub2web.metastore.ingenta.coms/"/brMethods.Nineobeserhesusmonkeyswerestudiedbyuseof18laparoscopicprocedures(twoprocedureseach,approx.sixweeksapart).Time-sensitiveliverandomentalATspecimenswereobtainedfrommonkeysundergeneralanesthesia,usingathree-portapproachwitharoticulatingendoscopicstapler/dividerandamonopolarelectrosurgeryforhemostasis.brxmlns="http://pub2web.metastore.ingenta.coms/"/brResults.Allsubjectstoleratedtheinitialandrepeatlaparoscopicprocedureswell.Liverspecimensweighedamean±SEMof3.8±0.5g,andomentalATspecimensweighed16.6±0.8g.Comparedwithpreviousstudiesofconventionallaparotomywithliverwedgeresection,themonkeysexperiencedfasterpostoperativerecoveryvialaparoscopy,withrapidreturntonormalfoodintakeandactivity.Minimaltonoadhesionswereobservedbyuseoftherepeatprocedureinallmonkeys,withnomajorcomplications.brxmlns="http://pub2web.metastore.ingenta.coms/"/brConclusions.Laparoscopyinobeserhesusmonkey(rangingfromyoungtoolder-aged),withrepeatedintra-abdominalliverandomentalATbiopsy,wasanexcellentminimallyinvasivesurgicalmethod.Incontrasttolaparotomywithwedgeresection,thisapproachgreatlydecreasesoperativetimeandstress,providesgeneroustissuespecimensinatime-efficientmanner,andfacilitatesrapidandfullrecoveryofthenonhumanprimate.