Characterization of emHaemophilus parasuis/em Serovar 2 CL120103, a Moderately Virulent Strain in China

摘要

emHaemophilus parasuis/em is an important bacterium affecting pigs, causing Gl?sser’s disease. To further characterize this species, we determined the complete genomic sequence of emH. parasuis/em CL120103, which was isolated from diseased pigs. The strain emH. parasuis/em CL120103 was identified as serovar 2. The size of the largest scaffold is 2,326,318 bp and contains 145 large contigs, with the N50 contig being 20,573 bp in length. The complete genome of emH. parasuis/em CL120103 is 2,305,354 bp in length with 39.97% GC content and contains 2227 protein-coding genes, 19 ribosomal rRNA operons and 60 tRNA genes. Sequence similarity of the genome of emH. parasuis/em CL120103 to the previously sequenced genome of emH. parasuis/em was up to 96% and query cover to 86%. Annotation of the genome of emH. parasuis/em CL120103 identified a number of genes encoding potential virulence factors. These virulence factors are involved in metabolism, adhesion, secretion and LPS biosynthesis. These related genes pave the way to better understand mechanisms underlying metabolic capabilities. The comprehensive genetic and phylogenetic analysis shows that emH. parasuis/em is closely related to emActinobacillus pleuropneumoniae/em and provides a foundation for future experimental confirmation of the virulence and pathogen-host interactions in emH. parasuis/em. This article offers supplementary material which is provided at the end of the article.