Spatial and Spectral Mapping and Decomposition of Neural Dynamics and Organization of the Mouse Brain with Multispectral Optoacoustic Tomography

摘要

In traditional optical imaging, limited light penetrationconstrains high-resolution interrogation to tissuesurfaces. Optoacoustic imaging combines thesuperb contrast of optical imaging with deep penetrationof ultrasound, enabling a range of newapplications. We used multispectral optoacoustictomography (MSOT) for functional and structuralneuroimaging in mice at resolution, depth, and specificityunattainable by other neuroimaging modalities.Based on multispectral readouts, we computed hemoglobingradient and oxygen saturation changesrelated to processing of somatosensory signals indifferent structures along the entire subcorticalcorticalaxis. Using temporal correlation analysisand seed-based maps, we reveal the connectivitybetween cortical, thalamic, and sub-thalamic formations.With the same modality, high-resolution structuraltomography of intact mouse brain was achievedbased on endogenous contrasts, demonstratingnear-perfect matches with anatomical features revealedby histology. These results extend the limitsof noninvasive observations beyond the reach ofstandard high-resolution neuroimaging, verifyingthe suitability of MSOT for small-animal studies.