THE EVALUATION OF EXPERIMENTAL RESPIRATORY EXPOSURE METHODS, WITH BENTONITE PARTICLES, ON WISTAR RATS

摘要

In a view to evaluate the exposure methods to PM, in laboratory conditions weve performed an experimental study on Wistar rats using three types of pollutants exposure: intranasal (IN), intratracheal (IT) and whole-body exposure by ventilation (VE). The experimental study was realized on rats (Wistar strain, males). The pollution agent was represented by bentonite particles (O-77,19%, Si-13,6%, Al-6,01%, Fe-1,38%, Na-1,03%, Mg-0,3%, Ti-0,16%, Ca-0,16%, K-0,15%.) with a medium size under 10m. The laboratory evaluations of physiological or morphological damages induced by bentonite at respiratory level were performed by biochemical analyses of blood (serum oxidative stress markers), cytological examination of BALF (broncho-alveolar lavage fluid) and histopathological examination of lung tissue. The main objective of this study was to establish the most proper method that can be use in our laboratory and the method that can lead to significant results using paraclinical evaluations. The inflammatory pathways were activated by bentonite exposure in rats, all the inflammatory phenomenons being increase in the animals with chronically exposure comparatively with the single-exposure groups. The exposure methods (intranasal instillation, intratracheal delivery and exposure by ventilation) can be easily applied for animal testing models.