Optimization Method For Biomolecular Identification of Muscle Larva of Trichinella species

摘要

Trichinellosis is a food-borne parasitic zoonosis with a yearly incidence of about10,000 clinical cases worldwide. It is one of the most serious zoonotic diseases in Romania with morethan 28,000 human cases reported over the last 25 years. In this context Romania remains the countrywith the highest infestation with Trichinella from the word. The aim of the paper it is to identify thesimple repetitive sequences from Trichinella genus through a biomolecular analysis of isolates. Afterconducting the DNA extraction from 50-100 muscle larvae of the six selective strains (jackal, wolf 1,wolf 4, 7.97, 7.42 and 7.29) using the QIAamp DNA (Qiagen, Germany) the amplification withESV primers (forward and reverse) using the mi-Taq Mix Kit (Metabion, Germany) followed,obtaining in this manner a positive amplification for all the six strains taken into study, revealing alsothe method's efficiency in the biomolecular identification of Trichinella strains. The goal of thisresearch, in perspective, is to improve the DNA extraction from one LM, in order to reduce thechemical and biological reagents. The positive amplification of the genomic DNA from one larva mayhave favorable repercussions on the genetic variability analysis in the population of Trichinellastudied, which can be applied on various individuals belonging to the same population.