A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases

Amanda Ribeiro Martins Da Silva; Cintia Duarte De Freitas Milagre; Leticia Mara Lima Angelini; Lucas De Freitas Coli Rocco

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A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases

机译：用于区分腈水合酶和腈水解酶的高通量筛选测定

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摘要

A modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects.

机译：改进的基于pH变化的比色高通量筛选结合能够区分腈水解酶和腈水合酶的酰胺酶抑制剂。这种酶促筛选基于二元反应，适用于分级筛选项目的第一步。

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《Brazilian Journal of Microbiology》 |2015年第1期|共4页
作者
Amanda Ribeiro Martins Da Silva; Cintia Duarte De Freitas Milagre; Leticia Mara Lima Angelini; Lucas De Freitas Coli Rocco;
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1. A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases [J] . Angelini Leticia Mara Lima, Silva Amanda Ribeiro Martins da, Rocco Lucas de Freitas Coli, Revista de microbiologia . 2015,第1期

机译：用于区分腈水合酶和腈水解酶的高通量筛选测定
2. A simple, efficient and rapid screening technique for differentiating nitrile hydratase and nitrilase producing bacteria [J] . Ruchi Sahu, Anil Kumar Meghavarnam, Savitha Janakiraman Biotechnology Reports . 2019,第1期

机译：区分腈水合酶和腈水解酶的细菌的简单，高效，快速筛选技术
3. Synthesis of enantiomerically pure (S)-mandelic acid using an oxynitrilase-nitrilase bienzymatic cascade: a nitrilase surprisingly shows nitrile hydratase activity [J] . Mateo C, Chmura A, Rustler S, Tetrahedron, Asymmetry: The International Journal for Repid Publication on all Aspects of Asymmetry in Orgainc, Inorganic, Organometallic, Physical and Bio-Organic Chemistry . 2006,第3期

机译：使用羟腈水解酶-腈水解酶双酶级联反应合成对映体纯的（S）-扁桃酸：腈水解酶出乎意料地显示出腈水合酶的活性
4. Transformation of dichlobenil to 2,6-dichlorobenzamide (BAM) by soil bacteria harboring nitrile hydratases and amidases or nitrilases [C] . M.S. Holtze, R.K. Juhler, J. Aamand, European Symposium on Environmental Biotechnology(ESEB 2004); 20040425-20040428; Oostende; BE . 2004

机译：具有腈水合酶和酰胺酶或腈水解酶的土壤细菌将二氯苯腈转化为2,6-二氯苯甲酰胺（BAM）
5. A High-Throughput Screening Assay Based on TRFRET to Identify Inhibitors for T. brucei Kinases [D] . Islam, Zeba. 2018

机译：基于TRFRET的高通量筛选测定法鉴定T.Brucei激酶的抑制剂
6. A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases [O] . Leticia Mara Lima Angelini, Amanda Ribeiro Martins da Silva, Lucas de Freitas Coli Rocco, 2015

机译：区分腈水合酶和腈水解酶的高通量筛选方法
7. A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases [O] . Angelini, Leticia Mara Lima [UNESP], Silva, Amanda Ribeiro Martins da [UNESP], Rocco, Lucas de Freitas Coli [UNESP], 2015

机译：区分腈水合酶和腈水解酶的高通量筛选方法

A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases

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