HPTLC Methods for Determination of Gemifloxacin Mesylate in Rabbit Plasma

摘要

Aim: A new, simple, rapid, very sensitive and accurate high performance thin-layer chromatographic (HPTLC) method has been developed and validated for estimation of Gemifloxacin in rabbit plasma. Study Design: Validation study. Methodology: HPTLC was performed on silica gel 60F₂₅₄ plates with ethanol: ethyl acetate: hexane, 2:7:1 (v/v), as mobile phase. Densitometry scanning was performed in absorbance mode at λ =254 nm. Result: The R _F value was 0.21. The response was a linear function of concentration over the range 0.1–0.7μg mL?1 ( r 2=0.996). A maximum recovery of drug from plasma was obtained by using chloroform and glacial acetic acid. Mean extraction recovery was 80%. Intra-day and inter-day precision (% RSD) of the assay were in the range 1.19–2.85% and accuracy was 1.7-5.66% Conclusion: This method can be applied to pharmacokinetic studies in rabbit plasma.