The Screening of EST-SSR Markers Associated with Peel Color in Tomato Fruit

摘要

Aims: The color of tomato fruit is an important property in improving of tomato breed. So we screened the differences of yellow tomato peel and transparent tomato peel using EST-SSR markers, and it is in favour of the research of the tomato fruit color and tomato breeding. Study Design: Primers were designed from ESTs in which the repeat bases of SSR were more than 18bp, then tested in yellow peel and transparent peel of gene pools built by F₂ from E-95F8(♀) and Zhonghuajufeng8(♂). Place and Duration of Study: College of Biological Science and Technology, between February 2015 and March 2016. Methodology: The yellow peel gene pool and the transparent peel gene pool were built according to the trait segregation group of F₂, and then screened the differential bands by the primers. In the end, the differential bands were tested in the segregation group of F₂. Results: 250 primer pairs were designed. One (HML52) of 250 primer pairs had diversity between yellow peel and transparent peel of gene pools built by F₂ from E-95F8(♀) and Zhonghuajufeng8(♂). In the end, the primer pair of HML52 passed test, and genetic distance was 7.04 cM. Conclusion: The primer pair of HML52 of EST-SSR marker associated with peel color in tomato was found in this study, and the genetic distance of the primer pair was 7.04 cM.