Expression and characterization of a GH43 endo-arabinanase from Thermotoga thermarum

摘要

Background Arabinan is an important plant polysaccharide degraded mainly by two hydrolytic enzymes, endo-arabinanase and α-L-arabinofuranosidase. In this study, the characterization and application in arabinan degradation of an endo-arabinanase from Thermotoga thermarum were investigated. Results The recombinant endo-arabinanase was expressed in Escherichia coli BL21 (DE3) and purified by heat treatment followed by purification on a nickel affinity column chromatography. The purified endo-arabinanase exhibited optimal activity at pH?6.5 and 75°C and its residual activity retained more than 80% of its initial activity after being incubated at 80°C for 2?h. The results showed that the endo-arabinanase was very effective for arabinan degradation at higher temperature. When linear arabinan was used as the substrate, the apparent K _m and V _max values were determined to be 12.3?±?0.15?mg?ml?1 and 1,052.1?±?12.7?μmol?ml?1?min?1, respectively (at pH?6.5, 75°C), and the calculated k _cat value was 349.3?±?4.2?s?1. Conclusions This work provides a useful endo-arabinanase with high thermostability andcatalytic efficiency, and these characteristics exhibit a great potential for enzymatic conversion of arabinan.