Production of keratinase from a new strain of Pseudomonas aeruginosa gmp and its application for the removal of dyed keratin waste

摘要

Keratins are insoluble structural proteins of skin, hair, horn, wool and claws. It is resistant to the action of common proteolytic enzymes however keratin can be hydrolyzed by microorganisms that can produce keratinolytic enzyme or keratinase. The objective of this work was the production of keratinase and degradation of keratin from microorganism isolated from poultry soil in Trichy. The biochemical and molecular characterization study reveals that the bacterial strain is Pseudomonas aeruginosa gmp with accession number JX027506. The optimum temperature and pH for keratinase production was found to be 700C and 7.0. The time course for keratinase production was found to be around 6 days. Pseudomonas aueruginosa gmp could degrade waste dyed keratin in 12 days.