Antioxidant Effect of Silymarin During Non-Enzymatic Peroxidation of Rat Kidney Microsomes and Mitochondria

摘要

Cellular metabolic changes during kidney disease may induce higher production of oxygen radicals that play a significant role in the progression of renal damage and in the onset of important comorbidities. In this study, the effect of Silymarin (SM) on chemiluminescence of mitochondria and microsomes isolated from kidney rat was analyzed and reported. After incubation of mitochondria and microsomes in an Ascorbate (0.4 mM)-Fe++ (2.15 μM) system (180 min at 37°C), non-enzymatic peroxidation was observed and the total cpm/mg of protein originated from light emission (chemiluminescence) was lower in kidney mitochondria and microsomes obtained from SM group than the control group (without SM). Moreover, it was observed that the SM was reduced based on concentration (6.25, 12.5, 25 and 50 μg) of chemiluminescence, and the same was measured as total cpm. The analyses of chemiluminescence indicate that SM may act as antioxidant protecting rat kidney mitochondria and microsomes from peroxidative damage.