Optimal system of fast propagation for Tamarind cotyledon nodes via tissue culture

摘要

Using young tamarind cotyledon nodes as the test material, the effects of different basic culture media, hormone concentrations, and seedling states on bud induction and different hormones on root induction were studied. The results showed that the optimum medium for inducting the adventitious bud of tamarind is 6-BA 3.0 mg/l + NAA 0.5 mg/l. The most suitable seedling state for bud induction was the S3 seedling whose cotyledon exited the seed shell completely and whose true leaves were not open. The S3 seedling had the best adventitious bud induction rate of 100% and a proliferation coefficient of up to 4.1. In addition, the appropriate rooting medium was ?MS + IBA 2.0 mg/l.