北柴胡幼根、子叶和下胚轴组织培养研究

摘要

研究了北柴胡幼根、子叶和下胚轴愈伤组织诱导、分化的条件.结果表明,MS +2,4-D 1.0 mg/L+ KT0.1 mg/L的培养基最适合于幼根、下胚轴和子叶愈伤组织的诱导,其中,幼根和下胚轴愈伤组织诱导率高于子叶；在B5+ 6-BA 1.5 mg/L+ NAA 0.03 mg/L+ CH 250 mg/L的培养基中,下胚轴和子叶愈伤组织的分化率最高；北柴胡试管苗生根培养基为1/2 MS+ NAA 0.5 mg/L.%In this paper,the conditions for induction and differentiation of callus in young root, cotyledon and hypotocyl of Bupleurum chinese were studied. The results demonstrated that MS medium added 2,4-D 1.0 mg/L, KT O.lmg/L were the most suitable for callus induction of young root, cotyledon and hypotocyl, especially for cotyledon and hypotocyl, while the B5 media with 6-BA 1.5 mg/L, NAA 0.03 mg/L and CH 250 mg/L were the most effective in callus differentiation. 1/2 MS medium added NAA 0.5 mg/L was right for rooting.