Heterogeneity of the Population of Lymphoma NK/Ly and Leukemia L-1210 Cells According to the Carbohydrate Structure of Cell Surfaces: Inimunocytochemical Analysis of Lectin Binding

摘要

—This paper investigates the heterogeneity of surface carbohydrate determinants in a population of ascites cells of lymphoma NK/Ly and leukemia L-1210 according to the binding of lectins in lentil, wheat germs, peanut, and concavalin A. Bound lectins were detected by an indirect immunochemical method using obtained by the authors colloidal gold-labeled polyclonal antilectin antibodies with further development with silver acetate. Significant differences were detected in the binding of concavalin A between NK/Ly (67% Con A+) and L-1210 (7.2% ConA+) cells, while the difference in the binding of other lectins with both types of tumor cells were not significant. A relatively high percentage of PNA+ cells was recorded; it can indicate on a high degree of desialization of membrane glycoproteins.