Dynamic mesenchymal stem cells volumic seeding in a commercialized porous ceramic scaffold: a feasibility study

摘要

According to the seeding tests conducted in the present study, the evaluated commercialized porous scaffold does not seem to be well adapted for homogeneous volumetric cell colonization. Indeed, although the cells are able to cross it entirely, the seeded cells rate after perfusion remains below 40% and the cell viability rate decreases rapidly, even under perfusion velocities that have been shown to be harmless to the cells in previous studies (Wendt et al. 2003). Moreover, histological observations (data not presented here) have shown that a large majority of the seeded cells were located along the outer upper face of the scaffold (i.e. on the first face of the scaffold encountered by the cells), which seems to indicate that the alveolar structure does not promote a massive cellular volumetric penetration into the scaffolds. In addition to further experimental tests to try to understand these paradoxical results, it would then be interesting to conduct an in silico study to highlight the structural key parameters required to enhance a homogeneous cell seeding in large bone scaffolds.