Isolation of the <1000 Dalton Migrants from Food Packaging Materials by Size Exclusion Chromatography (SEC)

摘要

The method enables the isolation of components of up to 1000 D molecular weight from extracts of food packaging materials. It is proposed to use a poly-styrene/divinylbenzene packing with an average nominal pore size of 500 A and tetrahydrofuran as mobile phase. The 1000 D cut is calibrated with triarachin, since this was the component of lowest retention per unit of molecular weight. As size exclusion chromatography (SEC) actually separates by molecular size rather than weight, some overlapping with components of higher molecular weights must be accepted. In the worst case the resulting fraction may include components of up to almost 2000 D molecular weight.