Construction of human combinatorial antibody library and screening of monoclonal antibody Fabs to human immunofeficiency virus type Ⅰ

摘要

The heavy chain Fd genes and k chain genes of immunoglobulin were amplified by RT-PCR from PBL of three volunteer donors with HIV-positive. Phage antibody library was constructed with the Fd genes and k chain genes using pComb3 as vector. Three-round selection against coated pg120 Showed specific enrichment of phage antibodies. After the third round selection, 40 out of 50 clones Exhibited gp120 binding capacity.