Combinatorial phagemid library of single chain human antibody enriched antibodies against vaccinia virus recombinant phagemid DNA pHEN-2A8, contain unique GENE single-chain human antibodies that can neutralize vaccinia and cowpox virus, and artificial single-stranded human antibody 2A8, the ability to neutralize vaccinia and smallpox viruses COWS

摘要

1. u043au043eu043cu0431u0438u043du0430u0442u043eu0440u043du0430u00a0 u0444u0430u0433u043cu0438u0434u043du0430u00a0 library u043eu0434u043du043eu0446u0435u043fu043eu0447u0435u0447u043du044bu0445 antibodies, antibodies against the virus u043eu0431u043eu0433u0430u0449u0435u043du043du0430u00a0 u043eu0441u043fu043eu0432u0430u043au0446u0438u043du044b, in the form of a collection of cells, e.coli TG1, u043au0430u0436u0434u0430u00a0 of which contains u0444u0430u0433u043cu0438u0434u043du0443u044e dna that after u0442u0440u0430u043du0441u0444u0435u043au0446u0438u0438 u0444u0430u0433u043eu043c assistant u043c13u043au043e7 biosynthesis u043du0438u0442u0447u0430u0442u044bu0445 u0431u0430u043au0442u0435u0440u0438u043eu0444u0430u0433u043eu0432, each of whom expo u043du0438u0440u0443u0435u0442 on its surface a unique u043eu0434u043du043eu0446u0435u043fu043eu0447u0435u0447u043du043eu0435 antibody rights, u043fu0440u0435u0434u0441u0442u0430u0432u043bu00a0u044eu0449u0435u0435 a u0445u0438u043cu0435u0440u043du044bu0439 protein with u043eu0431u043eu043bu043eu0447u0435u0447u043du044bu043c protein pIII u043du0438u0442u0447u0430u0442u043eu0433u043e bacteriophage m13.u0441u043au043eu043du0441u0442u0440u0443u0438u0440u043eu0432u0430u043du043du0430u00a0 by random u043eu0431u044au0435u0434u0438u043du0435u043du0438u00a0 in vitro comprising recombinant dna u0444u0430u0433u043cu0438u0434u043du044bu0445 u0432u0430u0440u0438u0430u0431u0435u043bu044cu043du044bu0445 domains u0442u00a0u0436u0435u043bu044bu0445 chains with u0432u0430u0440u0438u0430u0431u0435u043bu044cu043du044bu043cu0438 domains. chains of immunoglobulins, genes which are based on the messenger rna from peripheral blood lymphocytes of people u0438u043cu043cu0443u043du0438u0437u0438u0440u043eu0432u0430u043du044bu0445 virus u043eu0441u043fu043eu0432u0430u043au0446u0438u043du044b.;2. u0440u0435u043au043eu043cu0431u0438u043du0430u043du0442u043du0430u00a0 u0444u0430u0433u043cu0438u0434u043du0430u00a0 dna pHEN - 2A8, u043eu0442u043eu0431u0440u0430u043du043du0430u00a0 from a collection of recombinant u0444u0430u0433u043cu0438u0434u043du044bu0445 dna 1, u0441u043eu0434u0435u0440u0436u0430u0449u0430u00a0 unique gene u043eu0434u043du043eu0446u0435u043fu043eu0447u0435u0447u043du043eu0433u043e antibodies, u0441u043fu043eu0441 u043eu0431u043du043eu0433u043e to neutralize the virus and smallpox u043eu0441u043fu043eu0432u0430u043au0446u0438u043du044b cows, u0445u0430u0440u0430u043au0442u0435u0440u0438u0437u0443u0435u0442u0441u00a0 the following characteristics: has the u043cu043eu043bu0435u043au0443u043bu00a0u0440u043du0443u044e of 3.46 mda and the security u043f.u043d.contains the genetic engineering techniques of artificial gene u043eu0434u043du043eu0446u0435u043fu043eu0447u0435u0447u043du043eu0433u043e antibodies rights which under the control of promoter or u043bu0430u043au0442u043eu0437u043du043eu0433u043e operon and in the cells of e. coli fusion u043eu0434u043du043eu0446u0435u043fu043eu0447u0435u0447u043du043eu0433u043e antibodies rights of u0445u0438u043cu0435u0440u043du043eu0433u043e protein u043eu0431u043eu043bu043eu0447u0435u0447u043du044bu043c protein p3 u0444u0430u0433u0430 u043c13u043au043e7 that can neutralize the virus u043eu0441u043fu043eu0432u0430u043au0446u0438u043d s and smallpox cows, consists of the following elements:;SfiI / NotI - vector fragment u0444u0430u0433u043cu0438u0434u044b pHEN2 (mrc, u0432u0435u043bu0438u043au043eu0431u0440u0438u0442u0430u043du0438u00a0) the size of the 4495 u043f.u043e. containing promoter lac - u043eu043fu0435u0440u043eu043du0430 e. coli, e. coli forces, forces u0444u0430u0433u0430 m13, u0441u0443u043fu0440u0435u0441u0441u0438u0440u0443u0435u043cu044bu0439 stop codon TAG, fra u0433u043cu0435u043du0442 gene protein pIII u0444u0430u0433u0430 m13, gene - lactamase (bla), SfiI / NotI - 763 sukhoy fragment size.with artificial gene u043eu0434u043du043eu0446u0435u043fu043eu0447u0435u0447u043du043eu0433u043e antibodies capable of neutralizing human virus and smallpox u043eu0441u043fu043eu0432u0430u043au0446u0438u043du044b cows, which u0432u0430u0440u0438u0430u0431u0435u043bu044cu043du044bu0439 domain u0442u00a0u0436u0435u043bu043eu0439 chain is connected with the u0432u0430u0440u0438u0430u0431u0435u043bu044cu043du044bu043c domain light chain immunoglobulin human dna sequences, u043au043eu0434u0438u0440u0443u044eu0449u0435u0439 flexible u043fu0435u043fu0442u0438u0434u043du044bu0439 linker ser (Gly4Ser) 2Ala ArgGlySerGly4Ser, and a u043du0443u043au043bu0435u043eu0442u0438u0434u043du0443u044e sequenceat the u0444u0438u0433.6 contains promoter lac - u043eu043fu0435u0440u043eu043du0430 e. coli, genetic marker, gene - lactamase (bla), u043eu043fu0440u0435u0434u0435u043bu00a0u044eu0449u0438u0439 sustainability u0442u0440u0430u043du0441u0444u043eu0440u043cu0438u0440u043eu0432u0430u043du043du044bu0445 u0444u0430u0433u043cu0438u0434u043eu0439 pHEN - 2A8 cells of bacteria to u0430u043cu043fu0438u0446u0438u043bu043bu0438u043du0443, unique sites u0443u0437u043du0430u0432u0430u043du0438u00a0 u044du043du0434u043eu043du0443u043au043bu0435u0430u0437u0430u043cu0438 restriction with the following coordinates: HindIII - 235, SfiI - 328, NotI - 1091, EcoRI - 2382.;3. artificial u043eu0434u043du043eu0446u0435u043fu043eu0447u0435u0447u043du043eu0435 antibody rights 2u04308 capable of neutralizing the virus and smallpox u043eu0441u043fu043eu0432u0430u043au0446u0438u043du044b cows selected from combinatorial u0444u0430u0433u043cu0438u0434u043du043eu0439 u0431u0438u0431u043bu0438u043eu0442u0435 ki 1 on a u0430u043cu0438u043du043eu043au0438u0441u043bu043eu0442u043du0443u044e sequence contained in u0444u0438u0433.6.