Establishment and application of both FLP and Cre site-specific recombination systems at the same position in the genome

摘要

both FRT-FRT and LoxP-LoxP sites that are the target sequences of site-specific recombinases have been constructed in a vector, called C4LFY, using the recombinant DNA technique. C4LFY also contains P elements, 2 exons and 1 intron of Drosophila yellow gene, yellow promoter and enhancers, and flanking DNA. Since C4LFY made use of two pairs of FRT and LoxP sites, this vector included two site-specific recombination systems.